JRC GMO-Amplicons | EURL GMFF

Record ID:	MN334533.1
Database:	nt	Go to original record
Description:	Sonchus leptocephalus chloroplast, complete genome.
Taxon:	Sonchus leptocephalus
Original size:	152406 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47230	47752	+	523 bp

Amplicon:

523 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcacgtttt ccgaaaacaa acaaaggttc
151   agaaagcgaa aatcaaaaag gataggtgca gagactcgat ggaagctgtt
201   ctaacgaatg gagttgattg tcttacgtta gtagaggaat ccttctatcg
251   aaacttcaga aagaaggata aacctatata cataatacag acataataca
301   gaagaattgt tgtcaatcga ttccatattg aagaaagaat cgaatattca
351   ttgatcaaac cattcactcc ataatctgat agatcttttg aagaaatgat
401   taatcggacg agaataaaga tagagtcccg ttctacatgt caataccggc
451   aacaatgaaa tttatagtaa gaggaaaatc cgtcgatttc aaaaatcgtg
501   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.35e-08
primerB	100.0	20 bp	0	0	523	504	2.35e-08

Match details