JRC GMO-Amplicons | EURL GMFF

Record ID:	MN313430.1
Database:	nt	Go to original record
Description:	Grielum grandiflorum plastid, complete genome.
Taxon:	Grielum grandiflorum
Original size:	158189 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49625	50205	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aatgaaaaaa
101   gggcaatcct gagccaaatc ctgttttacg agaatacaca aaggttcaga
151   aagtaagaat aaaaaaaagg ataggtgcag agactcaatg gaagctgttc
201   taataaatta aatgggagtg actgcctttg cgttggtaaa ggactttttc
251   tatggatatt gaaactatag agaaggataa acctatatac atatgcaatg
301   caaaagtatc tcaaaaatga cgacctgaat ccctattttc ttatttatat
351   gaaaaaaaaa attgttacga atcgattcca agttgaagaa aaaatagaat
401   attcattaat caaagcattc gctccatagt ctgatcgatc ttttaattga
451   taactgatta atcggacgag aataaagata gagtcctgtt ctacatgtca
501   ataccgacaa caatgaaatt tatagtaaga ggaaaatccg tcgacttaaa
551   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details