JRC GMO-Amplicons | EURL GMFF

Record ID:	MW970109.1
Database:	nt	Go to original record
Description:	Dracocephalum heterophyllum chloroplast, complete genome.
Taxon:	Dracocephalum heterophyllum
Original size:	150860 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46143	46710	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgacaaaa aggataggtg cagagactca atggaagctg ttctaacgaa
201   tggagttgac tgcgccggta gcgatagagg aatctttcca tggaaatttt
251   agaaaggatg aaggataaac gcatctattg aatactatat caaattttta
301   atgttggccc aaatctgttt tttttttatt ttcatagtaa ataaaaaaaa
351   gtgtgaattt atttcacgtt gaaaaaaaaa atagaatatt catcaactca
401   ttcactccat agtccggtag atcttttaaa gaactcatta atcggacgag
451   aataaagata gagtcccgtt ctacatgcta catgtcaata ctggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details