JRC GMO-Amplicons | EURL GMFF

Record ID:	MK988314.1
Database:	nt	Go to original record
Description:	Rheum nobile voucher cp20190524 chloroplast, complete genome.
Taxon:	Rheum nobile
Original size:	161707 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49981	50583	+	603 bp

Amplicon:

603 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aattacaaaa
101   atgggcaatc ctgagccaac tccttctttc caaaaggaag aataaaaaag
151   gataggtgca gagactcaat ggaagctgtt ctaacaaatg gagttgacga
201   tcttgcgtta ttataaaaat tcttccatcg aaactccaaa aaagatgaag
251   aataaaccta tacgcagacg tacttaaata taaatactat atataataac
301   tatatattat ataaaaaaat gaaattaaaa aatattgatg acgacccgca
351   ttttttttat gactatgaaa aaatagaaga attgttgtga agcgattcca
401   agttgaagaa agagtcgaat atgcgtttaa taaattaaag catttattcc
451   ccggtctgag agatcttttg aagaaccgat ccatcggatg agaatgaaga
501   tagagtccca ttctacgtgt caatcctgac aacaatgaaa tttatagtaa
551   gaggaaaatc cgtcgacttt ataaatcgtg agggttcaag tccctctatc
601   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	603	584	2.72e-08

Match details