JRC GMO-Amplicons | EURL GMFF

Record ID:	MW646089.1
Database:	nt	Go to original record
Description:	Amaranthus retroflexus voucher 20120 chloroplast, complete genome.
Taxon:	Amaranthus retroflexus
Original size:	150710 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47418	48012	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa ctcctttttc aaaagtaaaa aaaaaaaaat
151   acgtattcag aaagcaagaa taaaaaaaag gataggtgca gagactcaaa
201   ggaagctgtt ctaacaaatg gagttgattg tgctgggttg ttcgaaatta
251   atgacacttc gaatctattc tgtatttcta tttttcgaat ttttagatat
301   ttcttatttc tagataatat agaatatgaa attttctaga aataaaaatg
351   aatatgaaaa aatggaagaa tttttgtgac tctatttcaa gttgaaaaaa
401   gaatcaaata ttcatcaaat cattcactcc atagtctgat agatctttct
451   ttgaaagaac cggttaatcg gttactcgga cgagaataaa gatagagtcc
501   cattatacat gtcaatactg acaacaatga aatttatagt acgaggaaaa
551   tccgtcgact ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details