JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610096.1
Database:	nt	Go to original record
Description:	Tripsacum australe isolate TK174 chloroplast, partial genome.
Taxon:	Tripsacum australe
Original size:	140788 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49289	49820	+	532 bp

Amplicon:

532 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaagat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctaaaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataatata
251   ggttctttat tttattttta gaatgaaatt aggaatgatt atgaaataga
301   aaattcataa ttttttttag aattattgtg aatctattcc aatcaaatat
351   tgagtaatca aatccttcaa ttcattgttt tcgagatctt ttaattttta
401   aaagtggatt aatcggacga ggataaagag agagtcccat tctacatgtc
451   aatactgaca acaatgaaat ttctagtaaa aggaaaatcc gtcgacttta
501   taagtcgtga gggttcaagt ccctctatcc cc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	532	513	2.39e-08

Match details