JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610090.1
Database:	nt	Go to original record
Description:	Rhytachne rottboellioides isolate TK027 chloroplast, partial genome.
Taxon:	Rhytachne rottboellioides
Original size:	137047 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48763	49290	+	528 bp

Amplicon:

528 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctagaaccaa aaggaaaagg ataggtgcag agactctatg gaagctattc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataatata
251   ggttctttat tttattttta taatgaaatt aggaatgatt atgaaataga
301   aaattcataa tttttttttt agaattattg tgaatctatt ccaatcgaat
351   attgagtaat caaatccttc aattcattgt tttcgggatc tttttaaaag
401   tggattaatc ggacgaggat aaagagagag tcccattcta catgtcaata
451   ctgacaacaa tgaaatttct agtaaaagga aaatccgtcg actttataag
501   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	528	509	2.37e-08

Match details