JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610088.1
Database:	nt	Go to original record
Description:	Sorghastrum nutans isolate TK035 chloroplast, partial genome.
Taxon:	Sorghastrum nutans
Original size:	140906 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49380	49906	+	527 bp

Amplicon:

527 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttgttttt gaaaaaacaa gtggttctca
151   aactagaacc caaaggaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacgaat cgaagtaata acgattaatc acagaaccca tattataata
251   taggttcttt attttatttt tagaatgaaa ttaggaatga ttatgaaata
301   gaaaattcat aattttttta gaattattgt gaatctattc caatcgaata
351   ttgagtaatc aaatccttca attcattggt ttcgagatct tttaaaaagt
401   ggattaatcg gacgaggata aagagagagt cccattctac atgtcaatac
451   tgacaacaat gaaatttcta gtaaaaggaa aatccgtcga ctttataagt
501   cgtgagggtt caagtccctc tatcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	527	508	2.37e-08

Match details