JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610084.1
Database:	nt	Go to original record
Description:	Tripsacum dactyloides var. floridanum isolate TK109 chloroplast, partial genome.
Taxon:	Tripsacum dactyloides var. floridanum
Original size:	140959 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49449	49974	+	526 bp

Amplicon:

526 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaagat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   cccaaaggaa aaggataggt gcagagactc aatggaagct gttctaacga
201   atcgaagtaa taacgattaa tcacagaacc cacattataa tataggttct
251   ttattttatt tttagaatga aattaggaat gattatgaaa tagaaaattc
301   ataatttttt ttagaattat tgtgaatcta ttccaatcaa atattgagta
351   atcaaatcct tcaattcatt gttttcgaga tcttttaatt tttaaaagtg
401   gattaatcgg acgaggataa agagagagtc ccattctaca tgtcaatact
451   gacaacaatg aaatttctag taaaaggaaa atccgtcgac tttataagtc
501   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	526	507	2.37e-08

Match details