JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610070.1
Database:	nt	Go to original record
Description:	Hyperthelia dissoluta isolate TK204 chloroplast, partial genome.
Taxon:	Hyperthelia dissoluta
Original size:	139162 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47665	48192	+	528 bp

Amplicon:

528 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctttttgaa aaaacaagcg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga agtaataacg attaatcaca gaacccatat tataatatag
251   gttctttatt ttatttttag aatgaaattt ggaaggatta tgaagaggta
301   tagaaaattc ataatttttt tagaattatt gtgaatctat tccaatcgaa
351   tattgagtaa tcaaatcctt caattcattg ttttcgagat cttttaaaag
401   tggattaatc ggacgaggat aaagagagag tcccattcta catgtcaata
451   ctgacaacaa tgaaatttct agtaaaagga aaatccgtcg actttataag
501   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	528	509	2.37e-08

Match details