JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610064.1
Database:	nt	Go to original record
Description:	Tripsacum pilosum isolate TK173 chloroplast, partial genome.
Taxon:	Tripsacum pilosum
Original size:	140923 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49366	49892	+	527 bp

Amplicon:

527 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaagat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt gcaaactaaa
151   acccaaagga aaaggatagg tgcagagact caatggaagc tgttctaacg
201   aatcgaagta ataacgatta atcacagaac ccatattata atataggttc
251   tttatttgat tttgagaatg aaattaggaa tgattatgaa atagaaaatt
301   cataattttt tttagaatta ttgtgaatct attccaatca aatattgagt
351   aatcaaatcc ttcaattcat tgttttcgag atcttttaat ttggaaaagt
401   ggattaatcg gacgaggata aagagagagt cccattctac atgtcaatac
451   tgacaacaat gaaatttcta gtaaaaggaa aatccgtcga ctttataagt
501   cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	527	508	2.37e-08

Match details