JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610062.1
Database:	nt	Go to original record
Description:	Ischaemum rugosum isolate TK186 chloroplast, partial genome.
Taxon:	Ischaemum rugosum
Original size:	140014 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48360	48888	+	529 bp

Amplicon:

529 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctttttttga aaaaacaact ggttctcaaa
151   ctagaaccca aaggaaaagg aaggataggt gcagagactc aatggaagct
201   gttctaacga atcgaagtaa taacgattaa tcacagaacc catattataa
251   tataggttct ttattttatt tttagaatga aattaggaat gattatgaaa
301   tagaaaattc ataatttttt tagaattatt gtgaatctat tccaatcgaa
351   tattgagtaa tcaaatcctt caattcattc ttttcgagat cttttaaaaa
401   gtggattaat cggacgagga taaagagaga gtcccattct acatgtcaat
451   actgacaaca atgaaatttc tagtaaaagg aaaatccgtc gactttataa
501   gtcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	529	510	2.38e-08

Match details