JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610058.1
Database:	nt	Go to original record
Description:	Apluda mutica isolate TK100 chloroplast, partial genome.
Taxon:	Apluda mutica
Original size:	140556 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49156	49680	+	525 bp

Amplicon:

525 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ttagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataatata
251   ggttctttat ttttttttta gaatgaaatt aggaatgatt atgaaataga
301   aaattcataa tttttttaga attattgtga atctattcca atcgaatatt
351   gagtaatcaa atccttcaat tcattgtttt cgagatcttt taaaaagtgg
401   attaatcgga cgaggataaa gagagagtcc cattctacat gtcaatactg
451   acaacaatga aatttctagt aaaaggaaaa tccgtcgact ttataagtcg
501   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.36e-08
primerB	100.0	20 bp	0	0	525	506	2.36e-08

Match details