JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610057.1
Database:	nt	Go to original record
Description:	Oxyrhachis gracillima isolate TK303 chloroplast, partial genome.
Taxon:	Oxyrhachis gracillima
Original size:	140497 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49294	49819	+	526 bp

Amplicon:

526 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaag
101   gggcaatcct gagccaaatc cctgttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcac agaacccata ttataatata
251   ggttctttat tttattttta gaatgaaatt aggaatgatt atgaaataga
301   aaatttcata atttttttag aatttttgtg aatctattcc aatcgaatat
351   tgagtaatca aatccttcaa ttcattgttt tcgagatctt ttaaaaagtg
401   gattaatcgg acgaggataa agagagagtc ccattctaca tgtcaatact
451   gacaacaatg aaatttctag taaaaggaaa atccgtcgac tttataagtc
501   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	526	507	2.37e-08

Match details