JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610056.1
Database:	nt	Go to original record
Description:	Diheteropogon hagerupii isolate TK183 chloroplast, partial genome.
Taxon:	Diheteropogon hagerupii
Original size:	139112 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47438	47967	+	530 bp

Amplicon:

530 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggcaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatccc agaacccata ttataatata
251   ggttctttat tttattttta gaatgaaatt tggaaggatt atgaaataga
301   aaattaataa tttttttaga attattgtga atctattcca atcgaatatt
351   gagtaatcaa atccttcaat tcattgtttt cgagattttt ttttttttaa
401   agtggattaa tcggacgagg ataaagagag agtcccattc tacatgtcaa
451   tactgacaac aatgaaattt ctagtaaaag gaaaatccgt cgactttata
501   agtcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	530	511	2.38e-08

Match details