JRC GMO-Amplicons | EURL GMFF

Record ID:	MT610051.1
Database:	nt	Go to original record
Description:	Cymbopogon martinii isolate TK048 chloroplast, partial genome.
Taxon:	Cymbopogon martinii
Original size:	139839 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48461	48989	+	529 bp

Amplicon:

529 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagc ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaataac gattaatcaa agaacccata ttataatata
251   ggttctttat tttattttta gaatgaaatt aggaatgatt atgaaataga
301   aaattcataa ttttttgaga attattgtga atctattcca atcgaatatt
351   gagtaatcaa atccttcaat tcattgtttt caagatcttt aaaaaaaaaa
401   gtggattaat cggacgagga taaagagaga gtcccattct acatgtcaat
451   actgacaaca atgaaatttc tagtaaaagg aaaatccgtc gactttataa
501   gtcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	529	510	2.38e-08

Match details