JRC GMO-Amplicons | EURL GMFF

Record ID:	KC414321.1
Database:	nt	Go to original record
Description:	Salvia tesquicola isolate 511190056 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Salvia tesquicola
Original size:	915 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	571	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttgactgcgc cggtagagga ttccatggaa actttagaaa
251   ggatgaagga taaacacatc tattgaatac tatattcaaa ttaaatgatt
301   aatgttgacc cgaatctgtt tttttaatat gaaaatgaaa aaatggaaaa
351   ataggtgtga atttatttca cgttgaagaa taaatcgaat attcctcaat
401   tcattcactc catagtccga tagatctttt aaagaactta ttaatcggac
451   gagaataaag atagagtccc attctacatg ctacatgtca ataccggcaa
501   caatgaaatt tatggtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details