JRC GMO-Amplicons | EURL GMFF

Record ID:	KC414319.1
Database:	nt	Go to original record
Description:	Salvia sclarea isolate 511190054 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Salvia sclarea
Original size:	897 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	553	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgccggt agaggattcc atggaaactt tagaaaggat
251   gaaggataaa cacatctatt gaatactata ttaaatgatt aatgttgacc
301   cgaatctgtt tttttaatat gaaaatgaaa aaataggtgt gaatttattt
351   cacgttgaag aataaatcga atattcctca attcattcac tccatagtcc
401   gatagatctt ttaaagaact tattaatcgg acgagaataa agatagagtc
451   ccattctaca tgctacatgt caataccggc aacaatgaaa tttatggtaa
501   gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details