JRC GMO-Amplicons | EURL GMFF

Record ID:	KC414306.1
Database:	nt	Go to original record
Description:	Salvia przewalskii isolate 511190040 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Salvia przewalskii
Original size:	887 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	528	+	528 bp

Amplicon:

528 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaaaaa aggataggtg
151   cagagactca atggaagttg ttctaacaaa tggagttgac tgcgcgggta
201   gaggaatctt tccacggaaa cttagaaagg atgaaggata aacccaccta
251   ttgaatacta tatcaaatga ttaatgttgg cccgaatctg tttttttaat
301   atgaaaatgg aaaaatcgat gtgaatttat ttcacgttga agaataaatc
351   gaatattcat caactcattc actccatagt tcgatagatc ttttaaagaa
401   cttattaatc ggacgagaat aaagatagag tcccattcta catgtcaata
451   ccggcaacaa tgaaatttat agtaagagga aaatccgtcg actttaaaaa
501   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	528	509	2.37e-08

Match details