JRC GMO-Amplicons | EURL GMFF

Record ID:	KC414305.1
Database:	nt	Go to original record
Description:	Salvia przewalskii isolate 511190039 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Salvia przewalskii
Original size:	884 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	525	+	525 bp

Amplicon:

525 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagcccc tgttttctca aaaaaaagga taggtgcaga
151   gactcaatgg aagttgttct aacaaatgga gttgactgcg ccggtagagg
201   aatctttcca cggaaacttt agaaaggatg aaggataaac ccatctattg
251   agtactatat caaatgatta atgttggccc gaatctgttt ttttaatatg
301   aaaatggaaa aatcgatgtg aatttatttc acgttgaaga ataaatcgaa
351   tattcatcaa ctcattcact ccatagttcg atagatcttt taaagaactt
401   attaatcgga cgagaataaa gatagagtcc cattctacat gtcaataccg
451   gcaacaatga aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg
501   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.36e-08
primerB	100.0	20 bp	0	0	525	506	2.36e-08

Match details