JRC GMO-Amplicons | EURL GMFF

Record ID:	KC414282.1
Database:	nt	Go to original record
Description:	Salvia cyclostegia isolate 511190009 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Salvia cyclostegia
Original size:	888 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	529	+	529 bp

Amplicon:

529 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaaaaa aaggataggt
151   gcagagactc gatggaagtt gttctaacaa atggagttga ctgcgccggt
201   agaggaatct ttccacggaa actttagaaa ggatgaagga taaacccatc
251   tattgaatac tatatcaaat gattaatgtt ggcccgaatc tgttttttaa
301   tatgaaaatg gaaaaatcga tgtgaattta tttcacgttg aagaataaat
351   cgaatattca tcaactcatt cactccatag ttcgatagat cttttaaaga
401   acttattaat cggacgagaa taaagataga gtcccattct acatgtcaat
451   accggcaaca atgaaattta tagtaagagg aaaatccgtc gactttaaaa
501   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	529	510	2.38e-08

Match details