JRC GMO-Amplicons | EURL GMFF

Record ID:	KC414278.1
Database:	nt	Go to original record
Description:	Salvia azurea var. grandiflora isolate 511190003 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Salvia azurea var. grandiflora
Original size:	890 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	531	+	531 bp

Amplicon:

531 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaaaaa aaaggatagg
151   tgcagagact caatggaagt tgttctaaca aatggagttg actgcgccgg
201   tagaggaatc tttccacgga aactttagaa aggatgaagg ataaacccat
251   ctattgaata ctatatcaaa tgattaatgt tggcccgaat ctgttttttt
301   aatatgaaaa tggaaaaatc gatgtgaatt tatttcacgt tgaagaataa
351   atcgaatatt catcaactca ttcactccat agttcgatag atcttttaaa
401   gaacttatta atcggacgag aataaagata gagtcccatt ctacatgtca
451   atactggcaa caatgaaatt tatagtaaga ggaaaatccg tcgacttaaa
501   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.39e-08
primerB	100.0	20 bp	0	0	531	512	2.39e-08

Match details