Match details

Record ID: JQ958261.1
Database: nt Go to original record
Description: Globulostylis minor voucher Dessein et al 2876 (BR) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL(UAA)) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon: Globulostylis minor
Original size: 1712 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 832 1382 + 551 bp

Amplicon: 551 bp
1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    cccactaagt gataactttc aaattcagag aaaccctgga attaataaaa
101   aggggcaatc ctgagccaaa tcctgttttc cgaaaccaaa ggttcagaaa
151   gtgaaaaagg gataggtgca gagactcgat ggaagttgtt ctaacaaatg
201   gagttggctg cgttagtaaa gaaatctttc catcgaaaat tccgaaagga
251   taaaacgtat acgtattgaa tactatatca aatgattaat gacgactcga
301   ctgaatccgt attttttata tgaaaacgga agaattggtg tgaatagatt
351   ccacattgaa gaaagaatcg aatattcatt gattaaatga ttcactccat
401   agtctgatag atcttttcaa gaattgatta atcggacgag aataaagata
451   gagtcccgtt ctacatgtca atgtcggcaa caatgaaatt tatagtaaga
501   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
551   c 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.48e-08
primerB 100.0 20 bp 0 0 551 532 2.48e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.