JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ958251.1
Database:	nt	Go to original record
Description:	Vangueriella chlorantha voucher Dessein et al 1632 (BR) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL(UAA)) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Vangueriella chlorantha
Original size:	1720 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	821	1390	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcga tggaagttgt tctaacaaat
201   ggagttggct gcgttagtaa agaaatcttt gttagtaaag aaatctttcc
251   atcgaaaatt ccgaaaggat aaaacgtata cgtattgaat actatatcaa
301   atgattaatg acgactcgac tgaatccgta ttttttatat gaaaacggaa
351   gaattggtgt gaatagattc cacattgaag aaagaatcga atattcattg
401   attaaatgat tcactccata gtctgataga tcttttcaag aattgattaa
451   tcggacgaga ataaagatag agtcccgttc tacatgtcaa tgtcggcaac
501   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details