JRC GMO-Amplicons | EURL GMFF

Record ID:	JQ958221.1
Database:	nt	Go to original record
Description:	Afrocanthium lactescens voucher Kuchar 23003 (BR) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL(UAA)) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Afrocanthium lactescens
Original size:	1719 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	816	1374	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   attgaaaaat gaaaaaggga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttggctgcg ttagtagaga aatctttcta tcgaaaattc
251   cgaaaggata aaacgtatac gtattgaata ctatatcaaa tgattaatga
301   cgactcgact gaatctgtat tttttatatg aaaatggaag aattggtgtg
351   aatagattcc acattgaaga aagaatcgaa tattcattga ttaaatgatt
401   cactccatag tctgatagat cttttcaaga attgattaat cggacgagaa
451   taaagataga gtcccgttct acatgtcaat gtcggcaaca atgaaattta
501   tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details