JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047844.1
Database:	nt	Go to original record
Description:	Wrixonia schultzii voucher Bishop 86 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Wrixonia schultzii
Original size:	1433 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	608	1140	+	533 bp

Amplicon:

533 bp

1     cgaaatcggt agacgctacg gacttgtgat aactttcaaa ttcagagaaa
51    ccccggaatt aataaaaatg ggtaatcctg agccaaatcc cgttttctca
101   aaacaaaggt aaaggttcaa aaaacgaaaa aagggatagg tgcagagact
151   caatggaagc tgttctaaca aatggagttg actgtcctgg tagaggcatc
201   tttgcatgga aacttccgaa aggatgaagg ataaatgtat ctattgaata
251   ctatatcaaa tgattaatga tggtccgaat ctgtatttgt attttttaat
301   atgaaaaatg gaagaattgg tgtgaattga ttccacattg aagaaaaaat
351   cgaatattca gtcagcaaat gattcactcc atagtccgat agatctttta
401   aagaactgat taatcggacg agaataaaga tagagtccca ttctacatgt
451   caataccggc aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt
501   aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.40e-08
primerB	100.0	20 bp	0	0	533	514	2.40e-08

Match details