JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047843.1
Database:	nt	Go to original record
Description:	Westringia lucida voucher Orme 600 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Westringia lucida
Original size:	1518 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	626	1187	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct gggtatggaa
51    tcctactaag tgataacttt tcaaattcag agaaaccccg gaattaataa
101   aaaatgggta atcctgagcc aaatcccgtt ttctcaaaac aaaggtaaag
151   gttcaaaaaa cgaaaaaaaa aagggatagg tgcagagact caatggaagc
201   tgttctaaca aatggagttg actgcgttgg tagaggaatc tttgcatgga
251   aacttccgaa aggatgaagg ataaacgtat ctattgaata ctatatcaaa
301   tgattaatga tggtccgaat ctgtatttgt attttttaat atgaaaaatg
351   gaagaattgg tgtgaattga ttccacattg aagaaaaaat cgaacaaatg
401   attcactcca tagtccgata gatcttttaa agaactgatt aatcggatga
451   gaataaagat agagtcccat tctacatgtc aataccggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details