JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047842.1
Database:	nt	Go to original record
Description:	Westringia longifolia voucher Hind 5426 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Westringia longifolia
Original size:	1499 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	611	1168	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaaagg gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttgactg cgttggtaga ggaatctttg catggaaact
251   tccgaaagga tgaaggataa acgtatctat tgaatactat atcaaatgat
301   taatgatggt ccgaatctgt atttgtattt tttaatatga aaaatggaag
351   aattggtgtg aattgattcc acattgaaga aaaaatcgaa caaatgattc
401   actccatagt ccgatagatc ttttaaagaa ctgattaatc ggatgagaat
451   aaagatagag tcccattcta catgtcaata ccggcaacaa tgaaatttat
501   agtaagagga aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details