JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047834.1
Database:	nt	Go to original record
Description:	Prostanthera staurophylla voucher Wilson 73 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera staurophylla
Original size:	1477 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	612	1184	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat tctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaagga taggtgcaga gactcgacgg aagctgttct
201   aacaaatgga gttgactgcc ctggtagagg catctttgca tggaaacttc
251   cgaaaggatg aaggataaat gtatctattg aatactatat caaaatcaaa
301   tgattaatga tggtccgaat ctgtatttgt attttttaat atgaaaaatg
351   gaataattgg tgtgaattga ttccacattg aagaaaaaat cgaatattca
401   gtcagcaaat gattcactcc atagtccaat agatctttta aagaactgat
451   taatcggacg agaataaaga tagagtccca ttctacatgt caataccggc
501   aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt caaaatcgtg
551   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details