JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047830.1
Database:	nt	Go to original record
Description:	Prostanthera serpyllifolia voucher Wilson 141 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera serpyllifolia
Original size:	1473 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	613	1180	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgcc ctggtagagc catctttgca tggaaacttc
251   cgaaaggatg aaggataaat gtatctattg aatactataa tcaaatgatt
301   aatgatggtc cgaatctgta tttgtatttt ttaatatgaa aaatggaaga
351   attggtgtga attgattcca cattgaagaa aaaatcgaat attcagtcag
401   caaatgattc actccatagt ccaatagatc ttttaaagaa ctgattaatc
451   ggacgagaat aaagatagag tcccattcta catgtcaata ccggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details