JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047829.1
Database:	nt	Go to original record
Description:	Prostanthera sejuncta voucher Wilson 64 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera sejuncta
Original size:	1471 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	603	1174	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atccccgttt tctcaaaaca aaggtaacgg
151   ttcaaaaaac gaaaaaaaaa aaggataggt gcagagactc aatggaagct
201   gttctaacaa atggagttga ctgccctgat agagggatct ttgcatggaa
251   acttctgaaa ggatgaagga taaatgtatc tattgaatac tatatcaaat
301   gattaatgat ggtccgaatc tgtatttgta ttttttaata tgaaaaatgg
351   aagaattggt gtgaattgat tccacattga agaaaaaatc gaatattcag
401   tcagcaaatg attcactcca tagtccgata gatcttttaa agaactgatt
451   aatcggacga gaataaagat agagtcccat tctacatgtc aataccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details