JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047826.1
Database:	nt	Go to original record
Description:	Prostanthera saxicola voucher Wilson 68 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera saxicola
Original size:	1475 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	613	1182	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaaaag ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgact gccctggtag aggcatcttt gcatggaaac
251   ttccgaaagg atgaaggata aatgtatcta ttgaatacta tatcaaatga
301   ttaatgatgg tccgaatctg tatttgtatt ttttaatatg aaaaatggaa
351   gaattggtgt caattgattc cacattgaag aaaaaatcga atattcagtc
401   agcaaatgat tcactccata gtccaataga tcttttaaag aactgattaa
451   tcggacgaga ataaagatag agtcccattc tacatgtcaa taccggcaac
501   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details