JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047825.1
Database:	nt	Go to original record
Description:	Prostanthera rugosa voucher Wilson 172 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera rugosa
Original size:	1497 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	638	1204	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg nnnnnnnnnt ggatgagcct tggtatggaa
51    cctactaagt gataactttc aaattcagag aaaccccgga attaataaaa
101   atgggtaatc ctgagccaaa tcccgttttc tcaaaagaaa ggtaaaggtt
151   taaaaaacga aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgcc ctggtagagg catctttgca tggaaacttc
251   cgaaaggatg aaggataaat gtatctattg aatactatat caaatgatta
301   atgatggtcc gaatctgtat ttgtattttt taatatgaaa aatggaagaa
351   ttggtgtgaa ttgattccac attgaagaaa aaatcgaata ttcagtcagc
401   aaatgattca ctccatagtc caatagatct tttaaagaac tgattaatcg
451   gacgagaata aagatagagt cccattctac atgtcaatac cggcaacaat
501   gaaatttata gtaataggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details