JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047812.1
Database:	nt	Go to original record
Description:	Prostanthera nivea voucher Wilson 61 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera nivea
Original size:	1492 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	628	1199	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgact gccctggtag aggcatcttt gcatggaaac
251   ttccgaaagg atgaagaata aatgtatcta ttgaatacta tatcattaat
301   gattaatgat ggtccgaatc tgtatttgta ttttttaata tgaaaaatgg
351   aagaattggt gtgaattgat tccacattga agaaaaaatc gaatattcag
401   tcagcaaatg attcactcca tagtccaata gatcttttaa agaactgatt
451   aatcggacga gaataaagat agagtcccat tctacatgtc aataccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details