JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047797.1
Database:	nt	Go to original record
Description:	Prostanthera hirtula voucher Wilson 170 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera hirtula
Original size:	1466 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	605	1173	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttgactg ccctgataga ggcatctttg catggaaact
251   tccgaaagga tgaaggataa atgtatctat tgaatactat atcaaatgat
301   taatgatggt ccgaatctgt atttgtattt tttaatatga aaaatggaag
351   aattggtgtg aattgattcc acattgaaga aaaaatcgaa tattcaatca
401   gcaaatgatt cactccatag tccgatagat cttttaaaga actgattaat
451   cggacgagaa taaagataga gtcccattct acatgtcaat accggcaaca
501   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details