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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: EF010979.1
Database: nt Go to original record
Description: Silencing vector pSE12860S, complete sequence.
Taxon: Silencing vector pSE12860S
Original size: 6268 bp

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Method: QL-ELE-00-026 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of pat gene; bar gene (partim bar) (Debode et al., 2016).
Target:bar

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 4873 4929 + 57 bp
Amplicon: 57 bp 
1     acaagcacgg tcaacttccg tacgagccgc aggaagcagg agtggacgga
51    cgacctc

View other 15 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 1.00e-08
primerB 100.0 18 bp 0 0 57 40 3.37e-08
probe 100.0 13 bp 0 0 23 35 2.03e-05

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.