JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047795.1
Database:	nt	Go to original record
Description:	Prostanthera grylloana voucher Sage 1871 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera grylloana
Original size:	1471 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	613	1178	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaatcagag aaaccccgga attaataaaa
101   atgggtaatc ctgagccaaa tcccgttttc tcaaaacaaa ggtaaaggtt
151   caaaaaacga aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttgactgccc tggtagaggc atctttgcac ggaaacttcc
251   gaaaggatga aggataagtg tatctattga atactatatc aaatgattaa
301   tgatggtccg aatctgtatt tgtatttttt aatatgaaaa atggaagaat
351   tggtgtaaat tgattccaca ttgaagaaaa aatcgaatat tcagtcagca
401   aatgattcac tccatagtcc aatagatctt ttaaagaact gattaatcgg
451   acgagaataa agatagagtc ccattctaca tgtcaatacc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details