JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047793.1
Database:	nt	Go to original record
Description:	Prostanthera galbraithiae voucher Conn 5222 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera galbraithiae
Original size:	1474 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	605	1181	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacct caaaacaaag
151   gtaaaggttc aaaaaacgaa aaaaaaagga taggtgcaga gactcaatgg
201   aagctgttct aacaaatgga gttgactgcc ctgatagagg catctttgca
251   tggaaacttc cgaaaggatg aaggataaat gtatctattg aatactatat
301   caaatgatta atgatggtcc gaatctgtat ttgtattttt taatatgaaa
351   aatggaagaa ttggtgtgaa ttgattccac attgaagaaa aaatcgaata
401   ttcagtcagc aaatgattca ctccatagtc cgatagatct tttaaagaac
451   tgattaatcg gacgagaata aagatagagt cccattctac atgtcaatac
501   cggcaacaat gaaatttata gtaagaggaa aatccgtcga ctttaaaaat
551   cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details