JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047789.1
Database:	nt	Go to original record
Description:	Prostanthera discolor voucher Kennedy 776 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera discolor
Original size:	1486 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	627	1193	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaagaa aggtaaaggt
151   ttaaaaaacg aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgcc ctggtagagg catctttgca tggaaacttc
251   cgaaaggatg aaggataaat gtatctattg aatactatat caaatgatta
301   atgatggtcc gaatctgtat ttgtattttt taatatgaaa aatggaagaa
351   ttggtgtgaa ttgattccac attgaagaaa aaatcgaata ttcagtcagc
401   aaatgattca ctccatagtc caatagatct tttaaagaac tgattaatcg
451   gacgagaata aagatagagt cccattctac atgtcaatac cggcaacaat
501   gaaatttata gtaataggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

View other 10 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details