JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047788.1
Database:	nt	Go to original record
Description:	Prostanthera howelliae voucher Wilson 149 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera howelliae
Original size:	1448 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	599	1159	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttgactg ccctgataga ggcatctttg catggaaact
251   tccgaaggat aaatgtatct attgaatact atatcaaacg attaatgatg
301   gtccgaatct gtatttgtat tttttaatat gaaaaatgga agaattggtg
351   tgaattgatt ccacattgaa gaaaaaatcg aatattcagt cagcaaatga
401   ttcactccat agtccgatag atcttttaaa gaactgatta atcggacgag
451   aataaagata gagtcccatt ctacatgtca ataccggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details