JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047776.1
Database:	nt	Go to original record
Description:	Prostanthera aff. baxteri TCW-2013 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera aff. baxteri TCW-2013
Original size:	1519 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	630	1191	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttgactg ccctggtaga ggcatctttg catggaaact
251   tccgaaagga tgaaggataa atgtatctat tgaatactat atcattaatg
301   attaatgatg gtccgaatct gtatttgtat tttttaatat gaaaaatgga
351   agaattggtg tgaattgatt ccacattgaa gaaaaaatcg aatattcagt
401   cagcaaatga ttcactccat agtccattaa agaactgatt aatcggacga
451   gaataaagat agagtcccat tctacatgtc aataccggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details