JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047773.1
Database:	nt	Go to original record
Description:	Prostanthera althoferi voucher Law 57 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Prostanthera althoferi
Original size:	1457 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	595	1164	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgattgcc cttggtagag gcatctttgc atggaaactt
251   ccgaaaggat gaaggataaa tgtatctatt gaatactata tcattaatga
301   ttaatgatgg tccgaatctg tatttgtatt ttttaatatg aaaaatgtaa
351   gaattgatgt gaattgattc cacattgaag aaaaaatcga atattcagtc
401   agcaaatgat tcactccata gtccaataga tcttttaaag aactgattaa
451   tcggacgaga ataaagatag agtcccattc tacatgtcaa taccggcaac
501   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details