JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047772.1
Database:	nt	Go to original record
Description:	Microcorys tenuifolia voucher Anon. s.n. trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Microcorys tenuifolia
Original size:	1493 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	614	1162	+	549 bp

Amplicon:

549 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tggtaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgcg ttggtagagg aatctttgca tggaaacttc
251   cgaaaggata aacgtatcta ttgaatacta tatcaaatga ttaatgatgg
301   tccgaatctg tatttgtatt ttttaatatg aaaaatggaa gaattggtgt
351   gaatttattc cacattgaag aaaaaatcga acaaatgatt cactccatag
401   tccgatagat cttttaaaga actgattaat cggacgagaa taaagagaga
451   gtcccattct acatgtcaat accggcaaca atgaaattta tagtaagagg
501   aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	549	530	2.47e-08

Match details