JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047771.1
Database:	nt	Go to original record
Description:	Hemigenia purpurea voucher Wilson 122 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Hemigenia purpurea
Original size:	1502 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	621	1171	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tggtaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttgactg cgttggtaga ggaatctttg catggaaact
251   tccgaaagga taaacgtatc tattgaatac tatatcaaat gattaatgat
301   ggtccgaatc tgtatttgta ttttttaata tgaaaaatgg aagaattggt
351   gtgaattgat tccacattga agaaaaaatc gaacaaatga ttcactccat
401   agtccgatag atcttttaaa gaactgatta atcggacgag aataaagaga
451   gagtcccatt ctacatgtca ataccggcaa caatgaaatt tatagtaaga
501   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details