JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047770.1
Database:	nt	Go to original record
Description:	Hemiandra coccinea voucher Anon. s.n. trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Hemiandra coccinea
Original size:	1499 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	616	1172	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcccgtttt ctcaaaacaa aggtaaaggt
151   tcaaaaaacg aaaaaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgc gttggtagag gaatctttgc atggaaactt
251   ccgaaaggat gaaggataac cgtatctatt gaatactata tcaaatgatt
301   aatgatggtc cgaatctgta tttgtatttt ttaatatgaa aaatggaaga
351   attggtgtga attgattcca cattgaagaa aaaatcgaac aaatgattca
401   ctccatagtc cgatagatct tttaaagaac tgattaatcg gacgagaata
451   aagatagagt cccattctac atgtcaatac cggcaacaat gaaatttata
501   gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details