JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047769.1
Database:	nt	Go to original record
Description:	Dicrastylis lewellinii voucher Johnstone 881 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Dicrastylis lewellinii
Original size:	1519 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	624	1184	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggtttaaaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgcgttggta gaggaacctt tccatggaaa cttcagaaag
251   gatgaaggat aaacgtatct attgaatact atatcaaatg attaatgatg
301   gtccgaatct gtatttgtat tttttaatat aaaaaatgga agaattggtg
351   tgaattgatt ccacattgaa gaaaaaatcg aatattcatt cagcaaatga
401   ttcactccat agtccgatag atcttttaaa gaactgatta atcggacgag
451   aataaagata gagtcccatt ctacatgtca ataccggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details