JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047768.1
Database:	nt	Go to original record
Description:	Dicrastylis exsuccosa voucher Anon. s.n. trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Dicrastylis exsuccosa
Original size:	1541 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	650	1209	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggtttaaaa
151   aacgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgact gcgttggtag aggaaccttt ccatggaaac ttcagaaagg
251   atgaaggata aacgtatcta ttgaatacta tatcaaatga ttaatgatgg
301   tccgaatctg tatttgtatt ttttaatata aaaaatggaa gaattggtgt
351   gaattgattc cacattgaag aaaaaatcga atattcattc agcaaatgat
401   tcactccata gtccgatagt tcttttaaag aactgattaa tcggacgaga
451   ataaagatag agtcccattc tacatgtcaa taccggcaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details