JRC GMO-Amplicons | EURL GMFF

Record ID:	JX047765.1
Database:	nt	Go to original record
Description:	Brachysola halganiacea voucher Anon. s.n. trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Brachysola halganiacea
Original size:	1534 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	638	1206	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaaatt ggattgagcc ttggtatgga
51    aacctgctaa gtgataactt tcaaattcag agaaaccccg gaaattaata
101   aaaatgggca atcctgagcc aatcctgttt tatcaaaact caaaacaaag
151   gttcaaagaa cgaaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttgactg cgttggtaga ggaatctttc catggaaact
251   tcagaaagga tgaaggataa acgtatctat tgaatactat atcaaatgat
301   taatgatggt ccgaatcttt atttgtattt tttaatacga aaaatggaag
351   aattggtgtg aattgattcc acattgaaga aaaaatcgaa tattcattca
401   gcaaatgatt cactccatag tccgatagtt cttttaaaga actgattaat
451   cggacgagaa taaagataga gtcccattct acatgtcaat accggcaaca
501   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details