JRC GMO-Amplicons | EURL GMFF

Record ID:	MW853784.1
Database:	nt	Go to original record
Description:	Lilium nepalense chloroplast, complete genome.
Taxon:	Lilium nepalense
Original size:	152206 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45586	46194	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttataa tataaaacta caataaaaaa agataggtgc agagactcaa
201   tggaagctgt tctaacgaat gaagttgatt acgttgcgct ggtagccgga
251   atccttctat caaaattacg gagaggatgc ccaccctata tacgtatata
301   tacatactga catagtaaac gattgattaa tcgcggcccg aatccattat
351   aatatatata tatatgaaaa atttaagagt tattctaaat ctattccata
401   ttccaatcaa agtttaagga agaatcgaat attcagtgat caaattattc
451   attccagagt ctgtatagat ctttttaaaa actgattatt cggacgagaa
501   taaagagaga gtcccattct acgtgtcaat accgacaaca atgaaattta
551   tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg ttcaagtccc
601   tctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details