JRC GMO-Amplicons | EURL GMFF

Record ID:	MW201575.1
Database:	nt	Go to original record
Description:	Paralamium griffithii chloroplast, complete genome.
Taxon:	Paralamium griffithii
Original size:	152664 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47450	48012	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaatta taattagatt gagccttggt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttctc aaaacaaagg
151   ttcaaaaaac gaaataaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgc gttggtagag gaatcctttc cacggaaact
251   tcagaaagga tgaaggataa acgtatctat tgaatactat atcaaatgat
301   taatgatggc ccgaatctgt attttttaat atgaaaaatg gaagaattgg
351   tgtgaattga ttctacattg aagaaaaaat agaatattca ttcatcaaat
401   cattcactcc atagtccgat agatctttta aagaactgat taatcggacg
451   agaataaaga tagagtccca ttctacatgt caataccggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details